This book presents the latest research on Ambient Intelligence presented in the 16th International Symposium on Ambient Intelligence (ISAmI 2024). Interested researchers can find works on advanced applications of Context-Aware Intelligence and Sensing for Smart Environments; AI-Driven Applications and Decision Support in Clinical and Personalized Healthcare; as well as Advanced AI and Interactive Systems for Emerging Technological Domains, such as Large Language Models, Extended Reality or 5G/6G networks. The symposium was organized by the Université of Lille (France), the Universidade do Minho (Portugal), the National Institute of Informatics (Japan), the Università degli Studi di Genova (Italy), the University of Granada (Spain), the AIR Institute (Spain), and the University de Salamanca (Spain). This edition of the conference was hosted by the SMAC Team of the CRIStAL Lab of the University of Lille (France) from June 25th to 27th, 2025.