This book constitutes the refereed proceedings of the 53rd Applied Imagery Pattern Recognition Workshop, AIPR 2025, held in Washington, DC, USA, during October 13-14, 2025. The 41 papers included in this volume were carefully reviewed and selected from 60 submissions. They focus on Imagery-Related challenges in Generative AI and Large Language Models, highlighting the accelerating impact of generative technologies on imagery, video, and multimodal reasoning.