Bioinformatics Tools for Medical Research and Molecular Diagnostics is a student-friendly guide designed to introduce essential bioinformatics tools used in modern medicine and molecular diagnostics. Written for MBBS, BDS, B.Sc., and B.Tech students, the book systematically covers biological databases, sequence retrieval and submission, file formats, and core analysis tools such as BLAST, FASTA, ClustalW, and Needleman-Wunsch algorithms. It further explores protein structure analysis, molecular visualization, docking, in silico drug design, and the emerging role of artificial intelligence in bioinformatics. With its clear explanations and practical focus, this book bridges biology and computation, supporting effective learning, research, and clinical applications.